Idebenone-induced recovery of glycerol-3-phosphate and succinate oxidation inhibited by digitonin

0382969 - FGÚ 2013 RIV CZ eng J - Journal Article
Rauchová, Hana - Vokurková, Martina - Drahota, Zdeněk
Idebenone-induced recovery of glycerol-3-phosphate and succinate oxidation inhibited by digitonin.
Physiological Research. Roč. 61, č. 3 (2012), s. 259-265. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA ČR(CZ) GA303/09/0570; GA ČR(CZ) GPP303/10/P227; GA MŠMT(CZ) 1M0510
Institutional research plan: CEZ:AV0Z50110509
Keywords : hyperthyroid liver mitochondria * oxygen consumption rate * Coenzyme Q * Cytochrome c
Subject RIV: CE - Biochemistry
Impact factor: 1.531, year: 2012

We report the inhibition of glycerol-3-phosphate- and succinate-dependent oxygen consumption rates by digitonin treatment. Our results show that the inhibition of oxygen consumption rates is recovered by the addition of exogenous synthetic analog of coenzyme Q idebenone (hydroxydecylubiquinone) and cytochrome c. Glycerol-3-phosphate oxidation rate is recovered to 148% of control values, whereas succinate-dependent oxidation rate only to 68%. Our findings might indicate the different mechanism of the electron transfer from two flavoprotein-dependent dehydrogenases (glycerol-3-phosphate and succinate dehydrogenases) localized on the outer and inner face of the inner mitochondrial membrane, respectively
Permanent Link: http://hdl.handle.net/11104/0213048