GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions

0382270 - ÚOCHB 2013 RIV US eng J - Journal Article
Riedl, Jan - Ménová, Petra - Pohl, Radek - Orság, Petr - Fojta, Miroslav - Hocek, Michal
GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions.
Journal of Organic Chemistry. Roč. 77, č. 18 (2012), s. 8287-8293. ISSN 0022-3263. E-ISSN 1520-6904
R&D Projects: GA ČR GA203/09/0317; GA ČR GBP206/12/G151
Institutional support: RVO:61388963 ; RVO:68081707
Keywords : DNA * oligonucleotides * polymerase * nucleotides * fluorescent labeling
Subject RIV: CC - Organic Chemistry
Impact factor: 4.564, year: 2012

GFP-like 3,5-difluoro-4-hydroxybenzylideneimidazolinone and 3,5-bis(methoxy)-4-hydroxy-benzylideneimidazolinone labels were attached to dCTP through propargyl linker and the resulting labeled nucleotides were used for a facile enzymatic synthesis of oligonucleotide or DNA probes by polymerase-catalyzed primer extension. The labeled DNA probes exerted low fluorescence which was increased 2-3.2 times upon binding of a protein. The FBI label was also used for a time-resolved experiment monitoring a single-nucleotide incorporation followed by primer extension by Vent(exo-) polymerase.
Permanent Link: http://hdl.handle.net/11104/0212539