A novel synthetic route of the caspase 3 sensor for determination of caspase 3 based on Förster resonance energy transfer

0382242 - ÚIACH 2013 US eng A - Abstract
Lišková, Marcela - Klepárník, Karel - Pazdera, P. - Foret, František
A novel synthetic route of the caspase 3 sensor for determination of caspase 3 based on Förster resonance energy transfer.
ITP 2012 - 19th International Symposium, Exhibit & Workshops on Electro- and Liquid Phase-separation Techniques. Baltimore, 2012. L-146.
[ITP 2012. International Symposium, Exhibit & Workshops on Electro- and Liquid Phase-separation Techniques /19./. 30.09.2012-03.10.2012, Baltimore]
R&D Projects: GA ČR(CZ) GAP301/11/2055; GA ČR(CZ) GBP206/12/G014; GA ČR GAP206/11/2377; GA MŠMT(CZ) CZ.1.07/2.3.00/20.0182; GA TA ČR(CZ) TA 02010672
Institutional support: RVO:68081715
Keywords : Caspase 3 * apoptpsis * FRET
Subject RIV: CB - Analytical Chemistry, Separation

The sensor is based on the principle of Förster resonance energy transfer (FRET). In this system, an energy absorbed by a chromophore (donor) is transferred nonradiatively to a fluorophore (acceptor) by dipole-dipole coupling through a linking chain. quantum dots (QDs) with high extinction coefficients and sulphorhodamine B are used as donors and acceptors. The molecule of our sensor consists of three parts.
Permanent Link: http://hdl.handle.net/11104/0215940