0382141 - ÚMCH 2013 RIV US eng J - Journal Article
Přikryl, P. - Lenfeld, Jiří - Horák, Daniel - Tichá, M. - Kučerová, Z.
Magnetic bead cellulose as a suitable support for immobilization of .alpha.-chymotrypsin.
Applied Biochemistry and Biotechnology. Roč. 168, č. 2 (2012), s. 295-305. ISSN 0273-2289. E-ISSN 1559-0291
R&D Projects: GA ČR GA203/09/0857
Institutional research plan: CEZ:AV0Z40500505
Keywords : enzyme immobilization * human gastric juice * immobilized α-chymotrypsin
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 1.893, year: 2012 ; AIS: 0.465, rok: 2012
DOI: https://doi.org/10.1007/s12010-012-9772-y

Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol–gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of α-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. α-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown.
Permanent Link: http://hdl.handle.net/11104/0212449