Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)

0381851 - MBÚ 2013 RIV NL eng J - Journal Article
Strachotová, Dita - Holoubek, A. - Kučerová, Helena - Benda, Aleš - Humpolíčková, Jana - Váchová, Libuše - Palková, Z.
Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM).
Biochimica Et Biophysica Acta-Biomembranes. Roč. 1818, č. 9 (2012), s. 2126-2134. ISSN 0005-2736. E-ISSN 1879-2642
R&D Projects: GA ČR GA204/08/0718; GA MŠMT(CZ) LC06063; GA MŠMT(CZ) LC531
Institutional research plan: CEZ:AV0Z40400503
Institutional support: RVO:61388971
Keywords : Ammonium exporters Ato1p * Ato2p and Ato3p * FLIM-photobleaching technique
Subject RIV: CE - Biochemistry
Impact factor: 3.389, year: 2012

Each of the three plasma membrane Ato proteins is involved in ammonium signalling and the development of yeast colonies. This suggests that although these proteins are homologous, they do not functionally substitute for each other, but may form a functional complex. Here, we present a detailed combined FRET, FLIM and photobleaching study, which enabled us to detect interactions between Ato proteins found in distinct compartments of yeast cells. We thus show that the proteins Ato1p and Ato2p interact and can form complexes when present in the plasma membrane. No interaction was detected between Ato1p and Ato3p or Ato2p and Ato3p. In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein, namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p. (C) 2012 Elsevier B.V. All rights reserved
Permanent Link: http://hdl.handle.net/11104/0212226