Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses

0381636 - ÚMCH 2013 RIV NZ eng J - Journal Article
Širc, Jakub - Kubinová, Šárka - Hobzová, Radka - Stránská, D. - Kozlík, P. - Bosáková, Z. - Mareková, Dana - Holáň, Vladimír - Syková, Eva - Michálek, Jiří
Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses.
International Journal of Nanomedicine. Roč. 7, 8 October (2012), s. 5315-5325. E-ISSN 1178-2013
R&D Projects: GA AV ČR KAN200520804
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z50520514; CEZ:AV0Z50390512
Institutional support: RVO:61389013 ; RVO:68378050 ; RVO:68378041
Keywords : nanofibers * electrospinning * multilayered structure
Subject RIV: CD - Macromolecular Chemistry; EI - Biotechnology ; Bionics (UMG-J); FH - Neurology (UEM-P)
Impact factor: 3.463, year: 2012

Polyvinyl alcohol nanofibers incorporating the wide spectrum antibiotic gentamicin were prepared by Nanospider™ needleless technology. A polyvinyl alcohol layer, serving as a drug reservoir, was covered from both sides by polyurethane layers of various thicknesses. The multilayered structure of the nanofibers was observed using scanning electron microscopy, the porosity was characterized by mercury porosimetry, and nitrogen adsorption/desorption measurements were used to determine specific surface areas. The stability of the gentamicin released from the electrospun layers was proved by high-performance liquid chromatography (HPLC) and inhibition of bacterial growth. Drug release was investigated using in vitro experiments with HPLC/MS quantification, while the antimicrobial efficacy was evaluated on Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Both experiments proved that the released gentamicin retained its activity and showed that the retention of the drug in the nanofibers was prolonged with the increasing thickness of the covering layers.
Permanent Link: http://hdl.handle.net/11104/0212059