Biophysical properties and cellular toxicity of covalent crosslinked oligomers of α-synuclein formed by photoinduced side-chain tyrosyl radicals

0381273 - BFÚ 2013 RIV US eng J - Journal Article
Borsarelli, C.D. - Falomir-Lockhart, L.J. - Ostatná, Veronika - Fauerbach, J.A. - Hsiao, H.-H. - Urlaub, H. - Paleček, Emil - Jares-Erijman, E.A. - Jovin, T.M.
Biophysical properties and cellular toxicity of covalent crosslinked oligomers of α-synuclein formed by photoinduced side-chain tyrosyl radicals.
Free Radical Biology and Medicine. Roč. 53, č. 4 (2012), s. 1004-1015. ISSN 0891-5849. E-ISSN 1873-4596
R&D Projects: GA AV ČR(CZ) KJB100040901
Institutional research plan: CEZ:AV0Z50040702
Keywords : Parkinson's disease * neurodegeneration * oxidative stress
Subject RIV: BO - Biophysics
Impact factor: 5.271, year: 2012

In this work, we describe the preparation and characterization of low molecular weight covalently bound oligomeric species of alpha-synuclein (aS) obtained by crosslinking via tyrosyl radicals. Numerous analytical techniques were used to characterize the aS oligomers. The diverse oligomeric species exhibited a direct effect on the in vitro aggregation behavior of wild-type monomeric aS.Compared to the unmodified monomer, the photoinduced covalent oligomeric species demonstrated increased toxic effects on differentiated neuronal-like SH-SY5Y cells. The results highlight the importance of protein modification induced by oxidative stress in the initial molecular events leading to Parkinson’s disease.
Permanent Link: http://hdl.handle.net/11104/0211785