Analysis of secretome and plasma using reversed phase liquid chromatography in mass spectrometry-based pipeline for enhanced biomarker development

0381256 - ÚIACH 2013 US eng A - Abstract
Šťastná, Miroslava - Van Eyk, J.E.
Analysis of secretome and plasma using reversed phase liquid chromatography in mass spectrometry-based pipeline for enhanced biomarker development.
ITP 2012 - 19th International Symposium, Exhibit & Workshops on Electro- and Liquid Phase-separation Techniques. Baltimore, 2012. s. 10.
[ITP 2012. International Symposium, Exhibit & Workshops on Electro- and Liquid Phase-separation Techniques /19./. 30.09.2012-03.10.2012, Baltimore]
Institutional support: RVO:68081715
Keywords : cardiac cell secretomes * reversed phase LC * mass spectrometry * proteomics
Subject RIV: CB - Analytical Chemistry, Separation

We used reversed phase LC under various experimental conditions to optimize preseparation of protein complex mixtures of cardiac myocyte secretomes and plasma prior to mass spectrometry identification. The effect of hypoxia on cardiac myocytes and fibroblasts was tested as well. The analysis of proteins secreted by various cells is an alternative approach to the direct analysis of plasma for discovery of potential protein biomarkers and therapeutic targets.
Permanent Link: http://hdl.handle.net/11104/0211772