0381085 - BÚ 2013 RIV US eng J - Journal Article
Krak, Karol - Janoušková, Martina - Caklová, Petra - Vosátka, Miroslav - Štorchová, Helena
Intraradical Dynamics of Two Coexisting Isolates of the Arbuscular Mycorrhizal Fungus Glomus intraradices Sensu Lato as Estimated by Real-Time PCR of Mitochondrial DNA.
Applied and Environmental Microbiology. Roč. 78, č. 10 (2012), s. 3630-3637. ISSN 0099-2240. E-ISSN 1098-5336
R&D Projects: GA ČR GA526/09/0838
Institutional support: RVO:67985939 ; RVO:61389030
Keywords : real-time PCR * mitochondrial DNA * coexistence
Subject RIV: EF - Botanics; EF - Botanics (UEB-Q)
Impact factor: 3.678, year: 2012 ; AIS: 1.359, rok: 2012
DOI: https://doi.org/10.1128/AEM.00035-12

We applied real-time PCR assays targeting the large subunit of mtDNA to monitor the DNA dynamics of two isolates of Glomus intraradices sensu lato coexisting in the roots of medic (Medicago sativa). The mtDNA-based quantification was compared to quantification in nrDNA. The ratio of copy numbers determined by the nrDNA- and mtDNA-based assays consistently differed between the two isolates. Within an isolate, copy numbers of the nuclear and the mitochondrial genes were closely correlated. The two quantification approaches revealed similar trends in the dynamics of both isolates, depending on whether they were inoculated alone or together. Taken together, the results of this study show that real-time PCR assays targeted to the large subunit of mtDNA may become useful tools for the study of coexisting AM fungi.
Permanent Link: http://hdl.handle.net/11104/0211631