Lysine(63)-linked ubiquitylation of PIN2 auxin carrier protein governs hormonally controlled adaptation of Arabidopsis root growth

0380702 - ÚEB 2013 RIV US eng J - Journal Article
Leitner, J. - Petrášek, Jan - Tomanov, K. - Retzer, K. - Pařezová, Markéta - Korbei, B. - Bachmair, A. - Zažímalová, Eva - Luschnig, Ch.
Lysine(63)-linked ubiquitylation of PIN2 auxin carrier protein governs hormonally controlled adaptation of Arabidopsis root growth.
Proceedings of the National Academy of Sciences of the United States of America. Roč. 109, č. 21 (2012), s. 8322-8327. ISSN 0027-8424. E-ISSN 1091-6490
R&D Projects: GA ČR(CZ) GAP305/11/2476
Institutional research plan: CEZ:AV0Z50380511
Keywords : PLASMA-MEMBRANE PROTEIN * EFFLUX CARRIER * INTRACELLULAR TRAFFICKING
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 9.737, year: 2012

Cross-talk between plant cells and their surroundings requires tight regulation of information exchange at the plasma membrane (PM), which involves dynamic adjustments of PM protein localization and turnover to modulate signal perception and solute transport at the interface between cells and their surroundings. In animals and fungi, turnover of PM proteins is controlled by reversible ubiquitylation, which signals endocytosis and delivery to the cell’s lytic compartment, and there is emerging evidence for related mechanisms in plants. Here, we describe the fate of Arabidopsis PIN2 protein, required for directional cellular efflux of the phytohormone auxin, and identify cis- and trans-acting mediators of PIN2 ubiquitylation. We demonstrate that ubiquitin acts as a principal signal for PM protein endocytosis in plants and reveal dynamic adjustments in PIN2 ubiquitylation coinciding with variations in vacuolar targeting and proteolytic turnover. We show that control of PIN2 proteolytic turnover via its ubiquitylation status is of significant importance for auxin distribution in root meristems and for environmentally controlled adaptations of root growth. Moreover, we provide experimental evidence indicating that PIN2 vacuolar sorting depends on modification specifically by lysine63-linked ubiquitin chains. Collectively, our results establish lysine63-linked PM cargo ubiquitylation as a regulator of polar auxin transport and adaptive growth responses in higher plants.
Permanent Link: http://hdl.handle.net/11104/0211344