The effect of endocrine disruptors on reproductive parameters and expression of selected testicular genes in male mice in vivo

0380388 - BTÚ 2013 DK eng A - Abstract
Pěknicová, Jana - Elzeinová, Fatima - Žatecká, Eva - Děd, Lukáš - Dorosh, Andriy
The effect of endocrine disruptors on reproductive parameters and expression of selected testicular genes in male mice in vivo.
American Journal of Reproductive Immunology. Roč. 67, Issue Supplement s1 (2012), s. 20. ISSN 1046-7408. E-ISSN 1600-0897.
[13th International Symposium for Immunology of reproduction "From the roots to the tops of Reproductive Immunology". 22.06.2012-24.06.2012, Varna]
R&D Projects: GA ČR(CZ) GA523/09/1793; GA ČR(CZ) GAP503/12/1834
Institutional research plan: CEZ:AV0Z50520701
Keywords : endocrine disruptors * testicular gene * reproductive parameters * reproduction
Subject RIV: DN - Health Impact of the Environment Quality

Problem. Endocrine disruptors (EDs) are exogenous substances that act like hormones, can disrupt the function of the endocrine systém, but their activity is not a result of physiological regulation. Many chemicals released into the environment can interfere with the action of the endocrine system through diverse mechanisms in animals and humans. The effect of low doses of selected EDs, xenoestrogens (bisphenol-A (BPA), p-nonylphenol (NP), diethylstilbestrol (DES)) and phytoestrogens (genistein (GEN), resveratrol (RES)), synthetic estrogenic hormone (17α-ethynylestradiol (EE2)) and polybrominated compound (tetrabromo-isopropylidenediphenol (TBBPA)) on the development of reproductive tract in outbred CD1 mice was tested. Materials and Methods. The effect of BPA, NP, DES, GEN, RES, EE2 and TBBPA on the body and organ weight, reproductive parameters, sperm quality and expression of selected genes was tested in a multigenerational study. Results. BPA and NP had a negative effect on the mouse offspring, on reproductive organ morphology and on the state of acrosome of mouse spermatozoa. This effect differed between the P and F1 generations. The fertility of mice exposed to DES was disrupted, especially in the first generation. Contrary to that, treatment by GEN and RES had no effect on the fertility of mice but had some effect on their reproductive parameters except for sperm integrity. Synthetic hormone (EE2) and polybrominated compound (TBBPA) also affected the organ weight, reproductive parameters, and sperm quality. TBBA changed the expression of selected genes encoding proteins that play key roles during spermatogenesis. Conclusion: We confirmed that all EDs affected the reproductive parameters and gene expression in male mice in vivo.
Permanent Link: http://hdl.handle.net/11104/0217285