Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes

0380149 - BFÚ 2013 RIV NL eng J - Journal Article
Ostatná, Veronika - Černocká, Hana - Kurzatkowska, K. - Paleček, Emil
Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes.
Analytica Chimica Acta. Roč. 735, JUL (2012), s. 31-36. ISSN 0003-2670. E-ISSN 1873-4324
R&D Projects: GA AV ČR(CZ) KJB100040901; GA ČR(CZ) GAP301/11/2055; GA MŠMT(CZ) ME09038
Institutional research plan: CEZ:AV0Z50040702
Keywords : protein denaturation * carbon electrodes * edge plane pyrolytic graphite
Subject RIV: BO - Biophysics
Impact factor: 4.387, year: 2012

In an attempt to develop a label-free electrochemical method for detection of changes in protein structures based on oxidizability of tyrosine and tryptophan residues we tested different types of carbon electrodes. We found that using edge plane pyrolytic graphite electrode we can discriminate between native and denatured forms of human serum albumin and of other proteins. The electrochemical denaturation curve agreed reasonably well with that based on intrinsic fluorescence of tyrosine and tryptophan. It can be expected that the electrochemical method will be applicable to a large number of proteins and may become useful in biomedicine and proteomics.
Permanent Link: http://hdl.handle.net/11104/0210937