Uptake and incorporation of sialic acid by the tick Ixodes ricinus

0379767 - BC 2013 RIV GB eng J - Journal Article
Vancová, Marie - Štěrba, J. - Dupejová, Jarmila - Simonová, Z. - Nebesářová, Jana - Novotný, M. V. - Grubhoffer, Libor
Uptake and incorporation of sialic acid by the tick Ixodes ricinus.
Journal of Insect Physiology. Roč. 58, č. 9 (2012), s. 1277-1287. ISSN 0022-1910. E-ISSN 1879-1611
R&D Projects: GA AV ČR KJB600960906; GA ČR GA206/09/1782; GA ČR GD206/09/H026; GA MŠMT(CZ) LC06009
Institutional research plan: CEZ:AV0Z60220518
Keywords : Tick * Ixodes ricinus * Sialic acid * Basement membrane * Mass spectrometry * Electron microscopy
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.379, year: 2012

We describe the detection of sialylated N-linked glycans in partially fed Ixodes ricinus tick females using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Sialylated glycans were detected in salivary glands as well as in tick guts and we propose the host origin of these structures. In addition, we mapped the transport of sialylated structures from the blood meal through the gut to the salivary glands using electron microscopy. Specific localization of sialylated glycans to basement membranes of salivary glands was observed. Finally, the influence of the sample preparation methods for electron microscopy on ultrastructure and immunogold labeling was evaluated.
Permanent Link: http://hdl.handle.net/11104/0210649