Development and characterization of multiplex panels of microsatellite markers for Syphacia obvelata, a parasite of the house mouse (Mus musculus), using a high throughput DNA sequencing approach

0379001 - ÚBO 2013 RIV NL eng J - Journal Article
Wasimuddin, Wasimuddin - Čížková, Dagmar - Ribas, Alexis - Piálek, Jaroslav - Goüy de Bellocq, Joëlle - Bryja, Josef
Development and characterization of multiplex panels of microsatellite markers for Syphacia obvelata, a parasite of the house mouse (Mus musculus), using a high throughput DNA sequencing approach.
Molecular and Biochemical Parasitology. Roč. 185, č. 2 (2012), s. 154-156. ISSN 0166-6851. E-ISSN 1872-9428
R&D Projects: GA ČR GA206/08/0640; GA MŠMT LC06073
Institutional support: RVO:68081766
Keywords : Microsatellites * Next-generation sequencing * Nematoda * Population genetics
Subject RIV: EG - Zoology
Impact factor: 2.734, year: 2012

Syphacia obvelata is a common gastro-intestinal parasitic nematode of the house mouse (Mus musculus), a prime model rodent species. Investigations of the genetic structure, variability of parasite populations and other biological aspects of this host–parasite system are limited due to the lack of genetic resources for S. obvelata. To fill this gap, we developed a set of microsatellite markers for S. obvelata, using a 454 pyrosequencing approach. We designed three multiplex panels allowing genotyping of 10 polymorphic loci and scrutinized them on 42 samples from two different regions inhabited by two different house mouse subspecies (Mus musculus musculus and M. m. domesticus). The numbers of alleles ranged from 2 to 6 with mean observed heterozygosities 0.1476 and 0.2095 for domesticus and musculus worms, respectively. The described markers will facilitate further studies on population biology and co-evolution of this host–parasite system.
Permanent Link: http://hdl.handle.net/11104/0210291