The Psb27 Assembly Factor Binds to the CP43 Complex of Photosystem II in the Cyanobacterium Synechocystis sp PCC 6803

0378820 - MBÚ 2013 RIV US eng J - Journal Article
Komenda, Josef - Knoppová, Jana - Kopečná, Jana - Sobotka, Roman - Halada, Petr - Yu, J. - Nickelsen, J. - Boehm, M. - Nixon, P. J.
The Psb27 Assembly Factor Binds to the CP43 Complex of Photosystem II in the Cyanobacterium Synechocystis sp PCC 6803.
Plant Physiology. Roč. 158, č. 1 (2012), s. 476-486. ISSN 0032-0889. E-ISSN 1532-2548
R&D Projects: GA ČR(CZ) GAP501/11/0377; GA ČR GAP501/10/1000; GA MŠMT LC07017
Institutional research plan: CEZ:AV0Z50200510
Keywords : MEMBRANE-PROTEIN COMPLEXES * SP PCC-6803 * D1 PROTEIN
Subject RIV: EE - Microbiology, Virology
Impact factor: 6.555, year: 2012

We have investigated the location of the Psb27 protein and its role in photosystem (PS) II biogenesis in the cyanobacterium Synechocystis sp. PCC 6803. Native gel electrophoresis revealed that Psb27 was present mainly in monomeric PSII core complexes but also in smaller amounts in dimeric PSII core complexes, in large PSII supercomplexes, and in the unassembled protein fraction. We conclude from analysis of assembly mutants and isolated histidine-tagged PSII subcomplexes that Psb27 associates with the "unassembled" CP43 complex, as well as with larger complexes containing CP43, possibly in the vicinity of the large lumenal loop connecting transmembrane helices 5 and 6 of CP43. A functional role for Psb27 in the biogenesis of CP43 is supported by the decreased accumulation and enhanced fragmentation of unassembled CP43 after inactivation of the psb27 gene in a mutant lacking CP47
Permanent Link: http://hdl.handle.net/11104/0210179