Challenges in analysis of cardiac cell secretomes by using pre-separation by RPLC and tandem mass spectrometry

0378038 - ÚIACH 2013 US eng A - Abstract
Šťastná, Miroslava - Goodliffe, L. - Simpson, J. - Van Eyk, J.E.
Challenges in analysis of cardiac cell secretomes by using pre-separation by RPLC and tandem mass spectrometry.
HPLC 2012: 38 th International Symposium on High Performance Liquid Phase Separations and Related Techniques. Anaheim, 2012. P-425-Tue.
[HPLC 2012: International Symposium on High Performance Liquid Phase Separations and Related Techniques /38./. 16.06.2012-21.06.2012, Anaheim]
Institutional support: RVO:68081715
Keywords : secreted proteins * cardiac cells * proteomics * mass spectrometry
Subject RIV: CB - Analytical Chemistry, Separation

We discuss various technical challenges and impediments involved in analysis of proteins secreted by cardiac cells into conditioned media in vitro by using RPLC followed by tandem mass spectrometry.
Permanent Link: http://hdl.handle.net/11104/0210025