Wolbachia and DNA barcoding Insects: Patterns, potential, and problems

0378011 - BC 2013 RIV US eng J - Journal Article
Smith, M. A. - Bertrand, C. - Crosby, K. - Eveleigh, E. S. - Fernandez-Triana, J. - Fisher, B. L. - Gibbs, J. - Hajibabaei, M. - Hallwachs, W. - Hind, K. - Hrček, Jan - Huang, D.-W. - Janda, M. - Janzen, D. H. - Li, Y. - Miller, S. E. - Packer, L. - Quicke, D. - Ratnasingham, S. - Rodriguez, J. - Rougerie, R. - Shaw, M. R. - Sheffield, C. - Stahlhut, J. K. - Steinke, D. - Whitfield, J. - Wood, M. - Zhou, X.
Wolbachia and DNA barcoding Insects: Patterns, potential, and problems.
PLoS ONE. Roč. 7, č. 5 (2012), e36514. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GAP505/10/0673
Grant - others:Marie Currie Fellowship(CZ) PIOFGA2009-25448; National Science Foundation(US) DEB 0072713; National Science Foundation(US) DEB 0344731; National Science Foundation(US) DEB 0842395; National Science Foundation(US) DEB 1020510; USDA(US) RC293-359; National Science Foundation(US) DEB 0841885; National Natural Science Foundation of China(CN) 31090253; National Science Foundation(US) EF-0553768; National Science Foundation(US) DEB 0515699
Institutional research plan: CEZ:AV0Z50070508
Institutional support: RVO:60077344
Keywords : Wolbachia
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.730, year: 2012
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036514

Wolbachia is a genus of bacterial endosymbionts that impacts the breeding systems of their hosts and can confuse the patterns of mitochondrial variation, including DNA barcodes. We examined the extent to which is Wolbachia detected in DNA barcoding, assessed its impact upon identification accuracy, and considered the variation present in Wolbachia COI. When two million insect COI trace files were examined on the Barcode of Life Datasystem, Wolbachia COI was present in 0.16% of the cases. It is possible to generate Wolbachia COI using standard insect primers, but that amplicon was never confused with host COI. Wolbachia alleles recovered were predominantly Supergroup A and were broadly distributed geographically and phylogenetically. The presence of the Wolbachia DNA in total genomic extracts made from insects is unlikely to compromise the accuracy of the DNA barcode library. Regular assays for Wolbachia presence and type should be adopted by large scale insect barcoding initiatives.
Permanent Link: http://hdl.handle.net/11104/0210003