Protective effect of Clostridium tyrobutyricum in acute dextran sodium sulphate-induced colitis: differential regulation of tumour necrosis factor-α and interleukin-18 in BALB/c and severe combined immunodeficiency mice

0377981 - MBÚ 2013 RIV GB eng J - Journal Article
Hudcovic, Tomáš - Kolínská, Jiřina - Klepetář, Jan - Štěpánková, Renata - Řezanka, Tomáš - Šrůtková, Dagmar - Schwarzer, Martin - Erban, V. - Du, Zhengyu - Wells, J. M. - Hrnčíř, Tomáš - Tlaskalová-Hogenová, Helena - Kozáková, Hana
Protective effect of Clostridium tyrobutyricum in acute dextran sodium sulphate-induced colitis: differential regulation of tumour necrosis factor-α and interleukin-18 in BALB/c and severe combined immunodeficiency mice.
Clinical and Experimental Immunology. Roč. 167, č. 2 (2012), s. 356-365. ISSN 0009-9104. E-ISSN 1365-2249
R&D Projects: GA ČR GA303/08/0367; GA ČR GA303/09/0449; GA ČR(CZ) GAP304/11/1252; GA MŠMT 2B06155; GA MŠMT(CZ) ME10017
Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z50110509
Keywords : butyrate * Clostridium tyrobutyricum * confocal fluorimetry
Subject RIV: EC - Immunology
Impact factor: 3.409, year: 2012

One of the promising approaches in the therapy of ulcerative colitis is administration of butyrate, an energy source for colonocytes, into the lumen of the colon. This study investigates the effect of butyrate producing bakterium Clostridium tyrobutyricum on dextran sodium sulphate (DSS)-induced colitis in mice. Immunocompetent BALB/c and immunodeficient severe combined immunodeficiency (SCID) mice reared in specific-pathogen-free (SPF) conditions were treated intrarectally with C. tyrobutyricum 1 week prior to the induction of DSS colitis and during oral DSS treatment. Administration of DSS without C. tyrobutyricum treatment led to an appearance of clinical symptoms – bleeding, rectal prolapses and colitis-induced increase in the antigen CD11b, a marker of infiltrating inflammatory cells in the lamina propria
Permanent Link: http://hdl.handle.net/11104/0209982