Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested native and glycated proteins

0377968 - FGÚ 2013 RIV DE eng J - Journal Article
Mikšík, Ivan - Lacinová, Kateřina - Zmatlíková, Zdeňka - Sedláková, Pavla - Král, V. - Sýkora, D. - Řezanka, P. - Kašička, Václav
Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested native and glycated proteins.
Journal of Separation Science. Roč. 35, č. 8 (2012), s. 994-1002. ISSN 1615-9306. E-ISSN 1615-9314
R&D Projects: GA ČR(CZ) GA203/09/0675; GA ČR(CZ) GA203/08/1428
Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z40550506
Keywords : capillary electrochromatography * gold nanoparticles * glycation * peptide maps * proteins
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.591, year: 2012

Open-tubular capillary electrochromatography (OT-CEC) method with bare gold nanoparticles-based stationary phase has been developed and applied for separation of tryptic peptide fragments of native and glycated proteins, bovine serum albumin, and human transferrin. The best separations of the above tryptic peptides were achieved in the BGE composed of aqueous 100 mmol/L sodium phosphate buffer, pH 2.5, at separation voltage 10 kV per 47-cm long, 50 um inside diameter capillary thermostated at 25 degrees C
Permanent Link: http://hdl.handle.net/11104/0209973