Type IV fimbrial subunit protein ApfA contributes to protection against porcine pleuropneumonia

0377903 - MBÚ 2013 RIV FR eng J - Journal Article
Sadílková, Lenka - Nepeřený, J. - Vrzal, V. - Šebo, Peter - Osička, Radim
Type IV fimbrial subunit protein ApfA contributes to protection against porcine pleuropneumonia.
Veterinary Research. Roč. 43, č. 2 (2012), s. 1-12. ISSN 0928-4249. E-ISSN 1297-9716
R&D Projects: GA MPO FT-TA2/025; GA AV ČR KAN200520702; GA ČR(CZ) GAP302/11/0580; GA ČR(CZ) GAP207/11/0717
Institutional research plan: CEZ:AV0Z50200510
Keywords : ApfA * immunogenicity * virulence factors
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.426, year: 2012

Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae accounts for serious economic losses in the pig farming industry worldwide. We examined here the immunogenicity and protective efficacy of the recombinant type IV fimbrial subunit protein ApfA as a single antigen vaccine against pleuropneumonia, or as a component of a multi-antigen preparation comprising five other recombinant antigens derived from key virulence factors of A. pleuropneumoniae (ApxIA, ApxIIA, ApxIIIA, ApxIVA and TbpB). Immunization of pigs with recombinant ApfA alone induced high levels of specific serum antibodies and provided partial protection against challenge with the heterologous A. pleuropneumoniae serotype 9 strain. This protection was higher than that engendered by vaccination with rApxIVA or rTbpB alone and similar to that observed after immunization with the tri-antigen combination of rApxIA, rApxIIA and rApxIIIA
Permanent Link: http://hdl.handle.net/11104/0209929