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High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
- 1.0377502 - MBÚ 2013 RIV NL eng J - Journal Article
Zawadzka-Kazimierczuk, A. - Kozminski, W. - Šanderová, Hana - Krásný, Libor
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins.
Journal of Biomolecular NMR. Roč. 52, č. 4 (2012), s. 329-337. ISSN 0925-2738. E-ISSN 1573-5001
R&D Projects: GA ČR GA204/09/0583
Institutional research plan: CEZ:AV0Z50200510
Keywords : Intrinsically disordered proteins * Non-uniform sampling * Backbone assignment
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.845, year: 2012
Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using delta subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences
Permanent Link: http://hdl.handle.net/11104/0209645
Number of the records: 1