Bovine serum albumin film as a template for controlled nanopancake and nanobubble formation: In situ atomic force microscopy and nanolithography study

0377465 - ÚFCH JH 2013 RIV NL eng J - Journal Article
Kolivoška, Viliam - Gál, Miroslav - Hromadová, Magdaléna - Lachmanová, Štěpánka - Tarábková, Hana - Janda, Pavel - Pospíšil, Lubomír - Morovská Turoňová, A.
Bovine serum albumin film as a template for controlled nanopancake and nanobubble formation: In situ atomic force microscopy and nanolithography study.
Colloids and Surfaces B-Biointerfaces. Roč. 94, JUN 1 2012 (2012), s. 213-219. ISSN 0927-7765. E-ISSN 1873-4367
R&D Projects: GA ČR GP203/09/P502; GA ČR GA203/09/0705; GA ČR GA203/08/1157; GA AV ČR IAA400400802
Institutional research plan: CEZ:AV0Z40400503
Keywords : protein adsorption * nanobubbles * nanopancakes
Subject RIV: CG - Electrochemistry
Impact factor: 3.554, year: 2012

Air nanobubbles and nanopancakes were investigated in situ by both tapping mode atomic force microscopy (TM AFM) and atomic force nanolithography techniques employing bovine serum albumin (BSA) film supported by highly oriented pyrolytic graphite (HOPG). The BSA denaturation induced by the water-to-ethanol exchange served for conservation of nanobubble and nanopancake sites appearing as imprints in BSA film left by gaseous cavities formerly present on the interface in the aqueous environment. Once the BSA film was gently removed by the nanoshaving technique applied in ethanol, a clean basal plane HOPG area with well-defined dimensions was regenerated. The subsequent reverse ethanol-to-water exchange led to the re-formation of nanopancakes specifically at the nanoshaved area. Our approach paves the way for the study of gaseous nanostructures with defined dimensions, formed at solid-liquid interface under controlled conditions.
Permanent Link: http://hdl.handle.net/11104/0209614