Molecular characterization of Atractolytocestus sagittatus (Cestoda: Caryophyllidea), monozoic parasite of common carp, and its differentiation from the invasive species Atractolytocestus huronensis

0377082 - BC 2013 RIV DE eng J - Journal Article
Bazsalovicsová, E. - Kráľová-Hromadová, I. - Štefka, Jan - Scholz, Tomáš
Molecular characterization of Atractolytocestus sagittatus (Cestoda: Caryophyllidea), monozoic parasite of common carp, and its differentiation from the invasive species Atractolytocestus huronensis.
Parasitology Research. Roč. 110, č. 5 (2012), s. 1621-1629. ISSN 0932-0113. E-ISSN 1432-1955
R&D Projects: GA ČR GA524/08/0885; GA MŠMT LC522
Institutional support: RVO:60077344
Keywords : INTERNAL TRANSCRIBED SPACER-1 * RIBOSOMAL-RNA GENES * SEQUENCE VARIATION * CYPRINUS-CARPIO * INTRAGENOMIC HETEROGENEITY * TAPEWORMS PLATYHELMINTHES * RDNA * EVOLUTION * POPULATION
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 2.852, year: 2012
http://link.springer.com/article/10.1007%2Fs00436-011-2673-0

Sequence structure of complete internal transcribed spacer 1 and 2 (ITS1 and ITS2) of the ribosomal DNA region and partial mitochondrial cytochrome c oxidase subunit I (cox1) gene sequences were studied in the monozoic tapeworm Atractolytocestus sagittatus (Kulakovskaya et Akhmerov, 1965) (Cestoda: Caryophyllidea), a parasite of common carp (Cyprinus carpio carpio L.). Intraindividual sequence diversity was observed in both ribosomal spacers. In ITS1, a total number of 19 recombinant clones yielded eight different sequence types (pairwise sequence identity, 99.7-100%) which, however, did not resemble the structure typical for divergent intragenomic ITS copies (paralogues). Polymorphism was displayed by several single nucleotide mutations present exclusively in single clones, but variation in the number of short repetitive motifs was not observed. In ITS2, a total of 21 recombinant clones yielded ten different sequence types (pairwise sequence identity, 97.5-100%). They were mostly characterized by a varying number of (TCGT)(n) repeats resulting in assortment of ITS2 sequences into two sequence variants, which reflected the structure specific for ITS paralogues. The third DNA region analysed, mitochondrial cox1 gene (669 bp) was detected to be 100% identical in all studied A. sagittatus individuals. Comparison of molecular data on A. sagittatus with those on Atractolytocestus huronensis Anthony, 1958, an invasive parasite of common carp, has shown that interspecific differences significantly exceeded intraspecific variation in both ribosomal spacers (81.4-82.5% in ITS1, 74.4-75.2% in ITS2) as well as in mitochondrial cox1, which confirms validity of both congeneric tapeworms parasitic in the same fish host.
Permanent Link: http://hdl.handle.net/11104/0209336