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Correcting the axial shrinkage of skeletal muscle thick sections visualized by confocal microscopy
- 1.0376810 - FGÚ 2013 RIV GB eng J - Journal Article
Janáček, Jiří - Kreft, M. - Čebašek, V. - Eržen, I.
Correcting the axial shrinkage of skeletal muscle thick sections visualized by confocal microscopy.
Journal of Microscopy. Roč. 246, č. 2 (2012), s. 107-112. ISSN 0022-2720. E-ISSN 1365-2818
R&D Projects: GA MŠMT(CZ) MEB090910; GA MŠMT(CZ) LC06063
Institutional research plan: CEZ:AV0Z50110509
Keywords : capillaries * confocal microscopy * sample deformation * shrinkage * skeletal muscle * 3D
Subject RIV: FH - Neurology
Impact factor: 1.633, year: 2012
We studied the deformation in thick sections of the rat skeletal muscle from complete stacks of images captured with confocal microscope. Careful sample preparation and treatment of the tissue cryosections with cold Ringer solution minimize the tissue deformation. Rescaling by the inverse of the axial scaling factor of the stack of optical slices in the direction of the microscope optical axis satisfactorily corrects the axial deformation of skeletal muscle samples
Permanent Link: http://hdl.handle.net/11104/0209120
Number of the records: 1