New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations

0376785 - ÚMCH 2013 RIV DE eng J - Journal Article
Horák, Daniel - Kučerová, J. - Korecká, L. - Jankovičová, B. - Palarčík, J. - Mikulášek, P. - Bílková, Z.
New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations.
Macromolecular Bioscience. Roč. 12, č. 5 (2012), s. 647-655. ISSN 1616-5187. E-ISSN 1616-5195
R&D Projects: GA ČR GA203/09/0857; GA ČR GAP503/10/0664; GA MŠMT 7E09109; GA MŠMT 7E12053
EU Projects: European Commission(XE) 246513 - NADINE; European Commission(XE) 228980 - CAMINEMS
Institutional research plan: CEZ:AV0Z40500505
Institutional support: RVO:61389013
Keywords : enzyme catalysis * glycidyl methacrylate * 2-hydroxyethyl methacrylate
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.742, year: 2012

Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.
Permanent Link: http://hdl.handle.net/11104/0006971