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The G-Patch Domain of Mason-Pfizer Monkey Virus Is a Part of Reverse Transcriptase
- 1.0376625 - ÚOCHB 2013 RIV US eng J - Journal Article
Křížová, Ivana - Hadravová, Romana - Štokrová, Jitka - Günterová, Jana - Doležal, Michal - Ruml, T. - Rumlová, Michaela - Pichová, Iva
The G-Patch Domain of Mason-Pfizer Monkey Virus Is a Part of Reverse Transcriptase.
Journal of Virology. Roč. 68, č. 4 (2012), s. 1988-1998. ISSN 0022-538X. E-ISSN 1098-5514
R&D Projects: GA MŠMT 1M0508; GA ČR GA204/09/1388
Grant - others:GA MŠk(CZ) ME 904
Institutional research plan: CEZ:AV0Z40550506
Keywords : DNA repair enzyme * retroviral protease * Toxoplasma gondii * leukemia-virus * containing RNA
Subject RIV: CE - Biochemistry
Impact factor: 5.076, year: 2012
Mason Pfizer monkey virus (M-PMV), like some other betaretroviruses, encodes a glycine-rich domain, G-patch domain (GPD). Alanine scanning mutational analysis of its most highly conserved residues revealed that while the mutations or deletions of the GPD had no effect on M-PMV proteolytic polyprotein processing, assembly and RNA incorporation, they caused a reduction of reverse transcriptase (RT) activity resulting in a drop in M-PMV infectivity. Immunoprecipitation experiments suggested that the GPD is a part of RT and participate in its function. These data indicate that the M-PMV GPD functions as a part of reverse transcriptase rather than protease.
Permanent Link: http://hdl.handle.net/11104/0208979
Number of the records: 1