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Genetic transformation of barley: limiting factors
- 1.0376035 - ÚEB 2013 RIV NL eng J - Journal Article
Vyroubalová, Š. - Šmehilová, M. - Galuszka, P. - Ohnoutková, Ludmila
Genetic transformation of barley: limiting factors.
Biologia Plantarum. Roč. 55, č. 2 (2011), s. 213-224. ISSN 0006-3134. E-ISSN 1573-8264
R&D Projects: GA ČR GD522/08/H003; GA MŠMT 1M06030
Institutional research plan: CEZ:AV0Z50380511
Keywords : Agrobacterium * albinism * Hordeum
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.974, year: 2011
This review summarizes main difficulties involved in barley (Hordeum vulgare L.) transformation. The most commonly used procedures for genetic transformation in barley are Agrobacterium tumefaciens and particle bombardment mediated methods. While different barley cultivars are used for genetic engineering with varying sensitivity, recent improvements in regeneration and transformation techniques are described and summarized. Furthermore, some of the transformation complicating factors, in particular somaclonal variation and transgene insertion sites, are discussed in more detail.
Permanent Link: http://hdl.handle.net/11104/0208543
Number of the records: 1