Characterization of the Drosophila adenosine receptor: the effect of adenosine analogs on cAMP signaling in Drosophila cells and their utility for in vivo experiments

0375936 - BC 2013 RIV GB eng J - Journal Article
Kučerová, Lucie - Brož, Václav - Fleischmannová, Jana - Šantrůčková, Eva - Sidorov, Roman - Doležal, Vladimír - Žurovec, Michal
Characterization of the Drosophila adenosine receptor: the effect of adenosine analogs on cAMP signaling in Drosophila cells and their utility for in vivo experiments.
Journal of Neurochemistry. Roč. 121, č. 3 (2012), s. 383-395. ISSN 0022-3042. E-ISSN 1471-4159
R&D Projects: GA ČR GAP305/10/2406; GA MŠMT(CZ) LC06077; GA MŠMT(CZ) LC554
Grant - others:European Community’s Seventh Framework Programme (FP7/2007-2013)(CZ) 229518; AV ČR(CZ) KJB501410801
Institutional research plan: CEZ:AV0Z50070508; CEZ:AV0Z50110509
Keywords : AdoR * calcium * CG9753
Subject RIV: CE - Biochemistry
Impact factor: 3.973, year: 2012
http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2012.07701.x/pdf

Adenosine receptors (AR) belonging to the G protein-coupled receptor family influence a wide range of physiological processes. Recent elucidation of the structure of human A2AR revealed the conserved amino acids necessary for contact with the Ado moiety. However, the selectivity of Ado analogs for AR subtypes is still not well understood. We have shown previously that the Drosophila adenosine receptor (DmAdoR) evokes an increase in cAMP and calcium concentration in heterologous cells. In this study, we have characterized the second-messenger stimulation by endogenous DmAdoR in a Drosophila neuroblast cell line and examined a number of Ado analogs for their ability to interact with DmAdoR. We show that Ado can stimulate cAMP but not calcium levels in Drosophila cells. We found one full and four partial DmAdoR agonists, as well as four antagonists. The employment of the full agonist, 2-chloroadenosine, in flies mimicked in vivo the phenotype of DmAdoR over-expression, whereas the antagonist, SCH58261, rescued the flies from the lethality caused by DmAdoR over-expression. Differences in pharmacological effect of the tested analogs between DmAdoR and human A2AR can be partially explained by the dissimilarity of specific key amino acid residues disclosed by the alignment of these receptors.
Permanent Link: http://hdl.handle.net/11104/0208466