The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells

0375923 - ÚMCH 2013 RIV NZ eng J - Journal Article
Babič, Michal - Horák, Daniel - Jendelová, Pavla - Herynek, V. - Proks, Vladimír - Vaněček, Václav - Lesný, Petr - Syková, Eva
The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells.
International Journal of Nanomedicine. Roč. 7, 16 March (2012), s. 1461-1474. E-ISSN 1178-2013
R&D Projects: GA ČR GA203/09/1242; GA MŠMT 1M0538; GA ČR GCP207/12/J013; GA ČR(CZ) GAP304/12/1370
EU Projects: European Commission(XE) 259796 - DIATOOLS
Institutional research plan: CEZ:AV0Z40500505; CEZ:AV0Z50390703
Keywords : nanoparticles * dopamine * hyaluronate
Subject RIV: FH - Neurology
Impact factor: 3.463, year: 2012

Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (γ-Fe2O3) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the γ-Fe2O3 surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/γ-Fe2O3 and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-γ-Fe2O3 nanoparticles with DPA-HA/γ-Fe2O3 = 0.45 (weight/weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells.
Permanent Link: http://hdl.handle.net/11104/0208459