Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1

0375145 - BC 2012 RIV DE eng J - Journal Article
Matoušek, Jaroslav - Patzak, J. - Kocábek, Tomáš - Füssy, Zoltán - Stehlík, Jan - Orctová, Lidmila - Duraisamy, Ganesh Selvaraj
Functional analyses of lupulin gland-specific regulatory factors from WD40, bHLH and Myb families of hop (Humulus lupulus L.) show formation of crucial complexes activating chs_H1.
Brewing Science. Roč. 64, č. 6 (2011), s. 151-155. ISSN 1613-2041
R&D Projects: GA ČR GA521/08/0740; GA MZe QH81052
Institutional research plan: CEZ:AV0Z50510513
Keywords : lupulin metabolome * Humulus lupulus L. * protein complexes * transcription factors
Subject RIV: EB - Genetics ; Molecular Biology

Complex lupulin gland-specific cDNA library from Osvald’s clone 72 hop was constructed to dissect regulation of biosynthetic pathway(s) leading to the accumulation of hop bitter acids and prenylflavonoids with promising health-beneficial activities. From this cDNA library we isolated first hop-specific allelic isoforms of transcription factors (TF) HlbHLH2 and HlWDR1 which are involved in combinatorial control of lightresponsive and tissue-specific activation of phenylpropanoid pathway. HlbHLH2 and HlWDR1 are quite lupulin gland-specific and, according to our transient expression experiments, they form specific complexes with another novel hop TF HlMyb2 and previously isolated lupulin glandspecific HlMyb3. The complex formation leads to strong activation of chalconsynthase gene (chs_H1) promoter. The interplay and regulation of expression of these crucial TF complexes could co-determine the rate of accumulation of valuable metabolites of lupulin.
Permanent Link: http://hdl.handle.net/11104/0207882