Toward single-molecule detection with sensors based on propagating surface plasmons

0374557 - ÚFE 2012 RIV US eng J - Journal Article
Kvasnička, Pavel - Chadt, Karel - Vala, Milan - Bocková, Markéta - Homola, Jiří
Toward single-molecule detection with sensors based on propagating surface plasmons.
Optics Letters. Roč. 37, č. 2 (2012), s. 163-165. ISSN 0146-9592. E-ISSN 1539-4794
R&D Projects: GA AV ČR KAN200670701; GA MŠMT OC09058; GA MŠMT(CZ) LH11102
Institutional research plan: CEZ:AV0Z20670512
Keywords : optical biosenzor * single molecule * surface plasmon microscopy
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 3.385, year: 2012

Surface plasmon resonance (SPR) sensors are known to be able to detect very low surface concentrations of (bio) molecules on macroscopic areas. To explore the potential of SPR biosensors for single-molecule detection, we have minimized the read-out area (to ~64 μm2) in a sensor generating SPs on a grating via a microscope objective and collecting light through a small aperture. Results suggest that as few as ~500 proteins could be detected by the sensor (3 orders of mag. lower than state-of-art).
Permanent Link: http://hdl.handle.net/11104/0207453