Surface plasmon resonance biosensor for the detection of VEGFR-1-a protein marker of myelodysplastic syndromes

0374555 - ÚFE 2013 RIV DE eng J - Journal Article
Pimková, K. - Bocková, Markéta - Hegnerová, Kateřina - Suttnar, J. - Čermák, J. - Homola, Jiří - Dyr, J. E.
Surface plasmon resonance biosensor for the detection of VEGFR-1-a protein marker of myelodysplastic syndromes.
Analytical and Bioanalytical Chemistry. Roč. 402, č. 1 (2012), s. 381-387. ISSN 1618-2642. E-ISSN 1618-2650
R&D Projects: GA AV ČR KAN200670701
Institutional research plan: CEZ:AV0Z20670512
Keywords : surface plasmon resonance * myelodysplastic syndromes * vascular endothelial growth factor
Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
Impact factor: 3.659, year: 2012

The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use.
Permanent Link: http://hdl.handle.net/11104/0207451