Analytical Value of Detecting an Individual Molecular Binding Event:The Case of the Surface Plasmon Resonance Biosensor

0374553 - ÚFE 2012 RIV US eng J - Journal Article
Šípová, Hana - Homola, Jiří
Analytical Value of Detecting an Individual Molecular Binding Event:The Case of the Surface Plasmon Resonance Biosensor.
Analytical Chemistry. Roč. 84, č. 1 (2012), s. 30-34. ISSN 0003-2700. E-ISSN 1520-6882
R&D Projects: GA AV ČR KAN200670701; GA MŠMT OC09058; GA MŠMT(CZ) LH11102
Institutional research plan: CEZ:AV0Z20670512
Keywords : surface plasmon resonance * single-molecule detection * kinetics * microfluidics
Subject RIV: JB - Sensors, Measurment, Regulation
Impact factor: 5.695, year: 2012

In this study, we compare the bioanalytical performance of flow-through surface plasmon resonance (SPR) sensors based on (1) localized surface plasmons (LSP) which detect a single binding event and (2) propagating surface plasmons (PSP) which integrate a great number of simultaneously occurring binding events. We demonstrate that for the biomolecular interactions most relevant to biosensing the LSP approach is inferior to the PSP approach due to low probability of positive sensor response.
Permanent Link: http://hdl.handle.net/11104/0207450