Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers

0373580 - MBÚ 2012 RIV NL eng J - Journal Article
Gryndler, Milan - Hršelová, Hana - Soukupová, Lucie - Streiblová, Eva - Valda, S. - Borovička, Jan - Gažo, J. - Miko, M.
Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers.
FEMS Microbiology Letters. Roč. 318, č. 1 (2011), s. 84-91. ISSN 0378-1097. E-ISSN 1574-6968
R&D Projects: GA ČR GAP504/10/0382
Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z30130516
Keywords : nested PCR * ITS primers * Tuber mesentericum
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.044, year: 2011

A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage was then used to distinguish the two species. The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field
Permanent Link: http://hdl.handle.net/11104/0206664