0373453 - BFÚ 2012 RIV US eng J - Journal Article
Paleček, Emil - Ostatná, Veronika - Černocká, Hana - Joerger, A.C. - Fersht, A.R.
Electrocatalytic monitoring of metal binding and mutation-induced conformational changes in p53 at picomole level.
Journal of the American Chemical Society. Roč. 133, č. 18 (2011), s. 7190-7196. ISSN 0002-7863. E-ISSN 1520-5126
R&D Projects: GA AV ČR(CZ) KAN400310651; GA AV ČR(CZ) KJB100040901; GA MŠMT(CZ) LC06035
Grant - others:GA ČR(CZ) GAP301/11/2055
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : p53 * conformational changes * constant current chronopotentiometric stripping (CPS)
Subject RIV: BO - Biophysics
Impact factor: 9.907, year: 2011 ; AIS: 2.799, rok: 2011
DOI: https://doi.org/10.1021/ja201006s

CPS on dithiothreitol-modified mercury electrodes enabled distinguishing of wt- and mutant of the tumor suppressor p53. Moreover, it was possible to monitor the loss of an essential zinc ion.
Permanent Link: http://hdl.handle.net/11104/0206550