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Chloroplast number estimation in mesophyll cells – comparison of disector and profile counting method
- 1.0373253 - FGÚ 2012 RIV IT eng O - Others
Kubínová, Z. - Lhotáková, Z. - Kubínová, Lucie - Albrechtová, J.
Chloroplast number estimation in mesophyll cells – comparison of disector and profile counting method.
Proceedings 10th Multinational Congress on Microscopy 2011. Urbino: Societa Italiana Scienze Micriscopiche, 2011. s. 385-386
R&D Projects: GA MŠMT(CZ) LC06063; GA ČR GAP501/10/0340
Institutional research plan: CEZ:AV0Z50110509
Keywords : chloroplast number * disector * stereology
Subject RIV: GK - Forestry
Two methods for chloroplast number estimation in mesophyll cells are compared and discussed. It is concluded that a frequently used method, based on counting chloroplast profiles in thin physical sections of the leaf is biased, resulting in a highly significant underestimation of the number of chloroplasts per cell. On the other hand, a stereological method of disector yields theoretically unbiased results
Permanent Link: http://hdl.handle.net/11104/0206381
Number of the records: 1