Number of the records: 1
Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94
- 1.0372330 - ÚVGZ 2012 RIV US eng J - Journal Article
Prudnikova, T. - Chaloupková, R. - Sato, Y. - Nagata, Y. - Degtjarik, O. - Kutý, Michal - Řezáčová, Pavlína - Damborský, J. - Kutá-Smatanová, Ivana
Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94.
Crystal Growth & Design. Roč. 11, č. 2 (2011), s. 516-519. ISSN 1528-7483. E-ISSN 1528-7505
R&D Projects: GA MŠMT(CZ) LC06010; GA MŠMT(CZ) ED1.1.00/02.0073
Institutional research plan: CEZ:AV0Z60870520; CEZ:AV0Z50520514
Keywords : crystal-structure * Japonicum * USDA110 * enzyme
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.720, year: 2011
The DbeA1 variant of a novel haloalkane dehalogenase DbeA (EC3.8.1.5) from Bradyrhizobium elkani USDA94 was constructed to study the structure-function relationships between DbjA and DbeA enzymes. A DbeA1 variant carries a unique fragment of nine amino acids transplanted from the sequentially closely related enzyme DbjA from Bradyrhizobium japonicum USDA 110 to DbeA. Here we report development of the crystallization protocol for DbeA1 and soaking experiments with the ligands 1-fluoropentane and 1,3-dichloropropane.
Permanent Link: http://hdl.handle.net/11104/0006846
Number of the records: 1