0371730 - ÚMG 2012 RIV US eng J - Journal Article
Nejepínská, Jana - Flemr, Matyáš - Svoboda, Petr
Control of the interferon response in RNAi experiments.
Methods in Molecular Biology. Roč. 820, - (2012), s. 133-161. ISSN 1064-3745
R&D Projects: GA AV ČR IAA501110701; GA ČR GA204/09/0085
Grant - others:EMBO SDIG(DE) 1483
Institutional research plan: CEZ:AV0Z50520514
Keywords : RNAi * interferon * siRNA
Subject RIV: EB - Genetics ; Molecular Biology

The RNA interference (RNAi) and interferons have been an uneasy marriage. Ever since the discovery of RNAi in mammals, the interferon response has been a feared problem. While RNAi became an efficient and widespread method for gene silencing in mammals, numerous studies recognized several obstacles, including undesirable activation of the interferon response, which need to be overcome to achieve a specific and robust RNAi effect. The aim of this text is to provide theoretical and practical information for scientists who want to control interferon response and other adverse effects in their RNAi experiments.
Permanent Link: http://hdl.handle.net/11104/0205179