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IL-12 inhibits the TGF-beta-dependent T cell developmental programs and skews the TGF-beta-induced differentiation into a Th1-like direction

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    0371356 - ÚMG 2012 RIV DE eng J - Journal Article
    Procházková, Jana - Pokorná, Kateřina - Holáň, Vladimír
    IL-12 inhibits the TGF-beta-dependent T cell developmental programs and skews the TGF-beta-induced differentiation into a Th1-like direction.
    Immunobiology. Roč. 217, č. 1 (2012), s. 74-82. ISSN 0171-2985
    R&D Projects: GA AV ČR KAN200520804; GA MŠMT 1M0506; GA ČR GAP304/11/0653; GA ČR(CZ) GAP301/11/1568; GA ČR GD310/08/H077
    Institutional research plan: CEZ:AV0Z50520514
    Keywords : cytokines * T cell differentiation * T cell subsets
    Subject RIV: EC - Immunology
    Impact factor: 2.814, year: 2012

    Development and differentiation of Th cells is highly plastic and strictly regulated by cytokines. We show negative regulation of TGF-beta-dependent differentiation programs by IL-12. Development of TGF-b-induced regulatory T cells (iTregs) or TGF-b/IL-6-activated Th17 cells from purified mouse CD4+CD25- T cells stimulated with anti-CD3 was abrogated by IL-12, establishing different developmental program. IL-12 inhibited expression of lineage-specific transcription factors Foxp3 and RORgt in developing Tregs and Th17 cells. IL-12 altered development of iTregs and Th17 cells even added after 48 h. Cells activated by TGF-b and IL-12 had increased expression of T-bet, produced Th1 cytokines IFN-g and IL-2 and expressed IL-18 receptor and C-C chemokine receptor type 5. Cells activated by both TGF-b and IL-12 stimulated macrophages to produce nitric oxide. IL-12 is shown as superior cytokine able to skew ongoing TGF-b-dependent iTreg or Th17 developmental program to Th1-like direction.
    Permanent Link: http://hdl.handle.net/11104/0204892

     
     
Number of the records: 1  

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