Expression and regulation of murine SPINK12, a potential orthologue of human LEKTI2

0371299 - ÚMG 2012 RIV US eng J - Journal Article
Reiss, K. - Meyer-Hoffert, U. - Fischer, J. - Sperrhacke, M. - Wu, Z. - Dimitrieva, O. - Křenek, Pavel - Suchanová, Šárka - Buryová, Halka - Brauer, Rena - Sedláček, Radislav
Expression and regulation of murine SPINK12, a potential orthologue of human LEKTI2.
Experimental Dermatology. Roč. 20, č. 11 (2011), s. 905-910. ISSN 0906-6705. E-ISSN 1600-0625
R&D Projects: GA ČR GC301/08/J053
Grant - others:Deutsche Forschungsgemeinschaft (DFG)(DE) Me2037/3-1
Institutional research plan: CEZ:AV0Z50520514
Keywords : epidermis * inhibitor * kallikrein * serine protease * spink
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.543, year: 2011

We describe expression and regulation of murine SPINK12, potential orthologue of human LEKTI2. Its expression was analysed by RT-PCR and immunohistochemistry revealing organ-specific pattern with high level of expression in epidermis and several epithelia including stomach, kidney and uterus. mSPINK12 expression in skin epidermis at footpads, where stratification is markedly pronounced, was several folds higher than in the abdominal epidermis. mSPINK12 mRNA levels were not affected by any cytokines tested while treatment of primary murine keratinocytes with the combination of calcium and sorbitol resulted in a strong increase in its mRNA. mspink12 seems especially expressed in the epidermal areas with thick skin and its regulation generally responds to differentiation signals. mrSPINK12 shows inhibitory activity against murine keratinocyte-derived trypsin-like proteolytic activity and appears orthologous to human LEKTI2, with possible role in regulation of epithelial cell functions.
Permanent Link: http://hdl.handle.net/11104/0204846