Critical evaluation of sample processing methods leading to improvement of immunogold labeling

0371069 - ÚMG 2012 IT eng A - Abstract
Sobol, Margaryta - Philimonenko, Vlada - Philimonenko, Anatoly - Hozák, Pavel
Critical evaluation of sample processing methods leading to improvement of immunogold labeling.
Proceedings, 10th Mutinational Congress on Microscopy 2011. Urbino: Italian Society of Microscopical Sciences, 2011. s. 125-126.
[Multinational Congress on Microscopy /10./. 04.09.2011-09.09.2011, Urbino]
R&D Projects: GA AV ČR KAN200520704; GA MŠMT 2B06063; GA MŠMT LC545
Institutional research plan: CEZ:AV0Z50520514
Keywords : cryofixation * AFS * resin embedding * histochemistry
Subject RIV: EB - Genetics ; Molecular Biology

Modern electron microscopy research demands appropriate stabilization of biological samples securing the best ultrastructural and antigenic preservation of molecular complexes. High-pressure freezing (HPF) of cells appears to be the method of primary choice for examining macromolecular assemblies in situ in close-to-native state leading to a better understanding of the functions of complex cellular structures [1]. Here we present results of our experiments aiming to improve the quality of biological samples in order to achieve an optimal preservation of both the ultrastructure and antigens before the subsequent immunogold labeling on ultrathin sections. All the described evaluations concern combinations of HPF and automated freeze substitution (FS) followed by resin embedment.
Permanent Link: http://hdl.handle.net/11104/0204699