Oxidation of sanguinarine and its dihydro-derivative at a pyrolytic graphite electrode using ex situ voltammetry. Study of the interactions of the alkaloids with DNA

0370858 - BFÚ 2012 RIV US eng J - Journal Article
Vacek, J. - Vrublová, E. - Kubala, M. - Janovská, M. - Fojta, Miroslav - Šimková, Eva - Stýskala, J. - Skopalová, J. - Hrbáč, J. - Ulrichová, J.
Oxidation of sanguinarine and its dihydro-derivative at a pyrolytic graphite electrode using ex situ voltammetry. Study of the interactions of the alkaloids with DNA.
Electroanalysis. Roč. 23, č. 7 (2011), s. 1671-1680. ISSN 1040-0397. E-ISSN 1521-4109
R&D Projects: GA MŠMT(CZ) LC06035
Grant - others:GA ČR(CZ) GPP503/11/P312
Program: GP
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : isoquinoline alkaloids * dihydrosanguinarine * voltammetry
Subject RIV: BO - Biophysics
Impact factor: 2.872, year: 2011

This study describes oxidation of sanguinarine (SG) and its metabolite dihydrosanguinarine (DHSG) on the surface of a basal-plane pyrolytic graphite electrode (PGE). Since both alkaloids strongly adsorb onto the surface of pyrolytic graphite, measurements were performed using ex situ voltammetric methods, adsorptive transfer (AdT) cyclic voltammetry (CV) and square-wave voltammetry (SWV). The electrochemical results and optimized AdT SWV were subsequently applied for study of the interactions of SG and DHSG with DNA in vitro. Electrochemical study of the interactions was supported and complemented with measurements using gel electrophoresis and fluorescence spectroscopy.
Permanent Link: http://hdl.handle.net/11104/0204540