Expression of osmotin, an antifungal protein from Nicotiana tabacum in Escherichia coli

0370645 - ÚOCHB 2012 CZ eng C - Conference Paper (international conference)
Viktorová, J. - Macková, M. - Macek, Tomáš
Expression of osmotin, an antifungal protein from Nicotiana tabacum in Escherichia coli.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 154-155. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
Grant - others:GA ČR(CZ) GAP501/11/1654
Program: GA
Institutional research plan: CEZ:AV0Z40550506
Keywords : pathogenesis-related proteins * osmotin * antifungal activity * recombinant protein
Subject RIV: CC - Organic Chemistry

Plants have evolved a huge variety of proteins involved in the defense against pathogens and adaptation to stressful environments. Plant proteins whose expression is strongly induced in response to infection by pathogens belong to the group of pathogenesis-related (PR) proteins. The family of PR-5 proteins constitutes a group of cysteine-rich proteins including thaumatin, zeamatin and also osmotin. Osmotin is a cationic protein of 205 residues and molecular weight of 24 kDa. It was discovered and characterized in cells of Nicotiana tabacum var. Wisconsin 38. The plasmid harbouring cDNA of osmotin from Nicotiana tabacum was constructed for transformation of Escherichia coli. The osmotin gene was prepared in fusion with histidine tail to facilitate the isolation and purification from bacterial cells. Selection of transgenic colonies was based on antibiotic resistance. The hexahistidine-tagged osmotin was overexpressed in heterologous system by using pET expression vector and purified using immobilized metal affinity chromatography. The expression of osmotin was detected and antifungal activity was tested.
Permanent Link: http://hdl.handle.net/11104/0204379