Study of antimicrobial peptide induction in Brassica napus

0370614 - ÚOCHB 2012 CZ eng C - Conference Paper (international conference)
Shevchenko, I. - Neubauerová, Tereza - Macková, Martina - Macek, Tomáš
Study of antimicrobial peptide induction in Brassica napus.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 127-129. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
Grant - others:GA ČR(CZ) GA522/09/1693; GA ČR(CZ) GD305/09/H008
Program: GA; GD
Institutional research plan: CEZ:AV0Z40550506
Keywords : Brassica napus * induced resistance * antimicrobial peptides
Subject RIV: CC - Organic Chemistry

Development of cheap, effective and environmentally friendly protection of industrial plants is a key problem of mankind, where using of antimicrobial peptides could be a reasonable solution. As a source for isolation of these peptides canola (Brassica napus) was chosen, which was treated by functional analog of salicylic acid – benzothiadiazole (BTH) – to stimulate the immune system. To isolate the responsible antimicrobial peptides, crude buffer extract was obtained. Next steps were precipitation, centrifugation and molecular weight filtration. The further isolation procedure comprised hexane extraction, SPE-C18 (Solid Phase Extraction) to separate hydrophobic and hydrophilic fractions and reversed-phase chromatography. Antimicrobial activity was tested by diffusion method. Fractions were tested on gram-positive and gram-negative bacteria and on yeast Candida scotii.
Permanent Link: http://hdl.handle.net/11104/0204354