Isolation and study of the properties of recombinant pseudoosmotin

0370597 - ÚOCHB 2012 CZ eng C - Conference Paper (international conference)
Richterová, K. - Nováková, Martina - Antošová, Z. - Neubauerová, Tereza - Macková, Martina
Isolation and study of the properties of recombinant pseudoosmotin.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 118-119. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
Grant - others:GA ČR(CZ) GAP501/11/1650
Institutional research plan: CEZ:AV0Z40550506
Keywords : osmotin * antifungal activity * antimicrobial peptides * recombinant proteins
Subject RIV: CC - Organic Chemistry

Antimicrobial peptides represent new clinical compounds with antimicrobial action and they could participate in solving the problem of ever-increasing resistance of microorganisms to the traditional antibiotics. The aim of this work was to study optimal conditions for expression, isolation and purification of recombinant pseudoosmotin from Escherichia coli BL21 (DE3). Pseudoosmotin was isolated from membrane fraction of bacteria and was purified by the affinity chromatography using Ni-NTA. Isolated pseudoosmotin did not show antimicrobial activity against studied microorganisms.
Permanent Link: http://hdl.handle.net/11104/0204347