Analysis of antimicrobial peptides by capillary electrophoresis

0370490 - ÚOCHB 2012 CZ eng C - Conference Paper (international conference)
Ehala, Sille - Niederhafner, Petr - Čeřovský, Václav - Řezanka, P. - Sýkora, D. - Král, V. - Kašička, Václav
Analysis of antimicrobial peptides by capillary electrophoresis.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 37-40. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
R&D Projects: GA ČR(CZ) GA203/09/0675; GA ČR(CZ) GA203/08/1428
Institutional research plan: CEZ:AV0Z40550506
Keywords : capillary electrophoresis * antimicrobial peptides * gold nanoparticles
Subject RIV: CC - Organic Chemistry

Capillary electrophoresis was used for the purity degree determination of new antimicrobial peptides: octadecapeptide melectin and tetradecapeptide mastoparan PDD-B, and their synthetic analogues. Besides, four semiempirical models correlating electrophoretic mobilities of these peptides with their charge and size (relative molecular mass) were applied to predict their probable structures in solution. Additionally, capillaries coated with gold nanoparticles were employed for the separation of mixtures of antimicrobial peptides.
Permanent Link: http://hdl.handle.net/11104/0204285