Peptide profilig of tryptic digests of water soluble proteins from Bacillus thuringiensis - transgenic and non-transgenic maize species by capillary zone electrophoresis

0370089 - ÚOCHB 2012 CZ eng C - Conference Paper (international conference)
Sázelová, Petra - Kašička, Václav - Ibanez, E. - Cifuentes, A.
Peptide profilig of tryptic digests of water soluble proteins from Bacillus thuringiensis - transgenic and non-transgenic maize species by capillary zone electrophoresis.
Biologically Active Peptides. 12th Conference. Praha: Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 - (Slaninová, J.), s. 123-126. Collection Symposium Series, 13. ISBN 978-80-86241-44-9.
[Biologically Active Peptides /12./. Praha (CZ), 27.04.2011-29.04.2011]
R&D Projects: GA ČR(CZ) GA203/08/1428; GA ČR(CZ) GA203/09/0675
Institutional research plan: CEZ:AV0Z40550506
Keywords : capillary zone electrophoresis * Bt-transgenic maize * water soluble proteins/peptides
Subject RIV: CC - Organic Chemistry

The tryptic digests of water-soluble proteins from transgenic Aristis-Bt and non-transgenic Aristis and Coventry maize varieties were analyzed by capillary zone electrophoresis (CZE) with UV-absorption detection at 206 nm in several acidic background electrolytes. Resolved tryptic peptides were characterized by effective electrophoretic mobilities and corrected peak areas. Some significant qualitative and quantitative differences in CZE-UV profiling of tryptic peptides were found between Aristis Bt and Aristis or between Aristis and Coventry varieties.
Permanent Link: http://hdl.handle.net/11104/0203993