Comparison of LC Columns Packed with 2.6 lm Core-Shell and Sub-2 lm Porous Particles for Gradient Separation of Antibiotics

0369992 - MBÚ 2012 RIV DE eng J - Journal Article
Tylová, Tereza - Kameník, Zdeněk - Flieger, Miroslav - Olšovská, Jana
Comparison of LC Columns Packed with 2.6 lm Core-Shell and Sub-2 lm Porous Particles for Gradient Separation of Antibiotics.
Chromatographia. Roč. 74, 1-2 (2011), s. 19-27. ISSN 0009-5893. E-ISSN 1612-1112
R&D Projects: GA MŠMT 1M06011
Institutional research plan: CEZ:AV0Z50200510
Keywords : Column liquid chromatography * Sub-2 mu m particles * Acquity BEH C18 column
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.195, year: 2011

The recently introduced Kinetex C18 column packed with core-shell 2.6 lm particles is declared to provide similar efficiency and short analysis as Acquity BEH C18 column with 1.7 lm porous particles. Unlike Acquity BEH C18 column, Kinetex C18 column exhibited lower column backpressure making this column compatible to conventional LC systems. The performance of Kinetex C18 column (2.1 9 50 mm) and Acquity BEH C18 column (2.1 9 50 mm) for gradient separation of tetracyclines under acidic conditions (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) and macrolides under alkaline conditions (tylosin, clarithromycin, roxithromycin, and carbomycin) was studied. The columns were compared by evaluation of their experimental peak capacity and its dependence on linear velocity and gradient slope
Permanent Link: http://hdl.handle.net/11104/0203920