Responses of Transgenic Tobacco Plants with Increased Proline Content to Drought and/or Heat Stress

0369947 - ÚEB 2012 RIV US eng J - Journal Article
Pospíšilová, Jana - Haisel, Daniel - Vaňková, Radomíra
Responses of Transgenic Tobacco Plants with Increased Proline Content to Drought and/or Heat Stress.
American Journal of Plant Sciences. Roč. 2, č. 3 (2011), s. 318-324. ISSN 2158-2742
R&D Projects: GA ČR GA522/09/2058
Institutional research plan: CEZ:AV0Z50380511
Keywords : Carotenoids * Chlorophyll * Net Photosynthetic Rate
Subject RIV: ED - Physiology
http://www.scirp.org/journal/PaperInformation.aspx?paperID=7576

Transgenic tobacco plants (M51-1) constitutively over-expressing a modified gene for the proline biosynthetic enzyme delta2-pyrroline-5-carboxylate synthetase (P5CSF129A) and the corresponding wild-type plants (WT) were compared during drought or heat stress and under combination of both stresses. The proline content in M51-1 was several times higher than in WT plants. Under optimal conditions, the transpiration rate and stomatal conductance of M51-1 plants were lower than those in WT plants. The differences in net photosynthetic rate were not significant and water use efficiency and contents of chlorophyll and xanthophyll cycle pigments were higher in M51-1 than in WT plants. Drought induced by cessation of watering for 7 d resulted in decrease of all gas exchange parameters and chlorophyll content, but in an increase of the content of xanthophyll cycle pigments and degree of their de-epoxidation.
Permanent Link: http://hdl.handle.net/11104/0203888