A major invasion of transposable elements accounts for the large size of the Blumeria graminis f.sp. tritici genome

0369945 - ÚEB 2012 RIV DE eng J - Journal Article
Parlange, Z. - Oberhaensli, S. - Breen, J. - Platzer, M. - Taudien, S. - Šimková, Hana - Wicker, T. - Doležel, Jaroslav - Keller, B.
A major invasion of transposable elements accounts for the large size of the Blumeria graminis f.sp. tritici genome.
Functional & Integrative Genomics. Roč. 11, č. 4 (2011), s. 671-677. ISSN 1438-793X. E-ISSN 1438-7948
R&D Projects: GA MŠMT ED0007/01/01
Institutional research plan: CEZ:AV0Z50380511
Keywords : Blumeria graminis * BAC library * BAC-end sequences
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.842, year: 2011

Powdery mildew of wheat (Triticum aestivum L.) is caused by the ascomycete fungus Blumeria graminis f.sp. tritici. Genomic approaches open new ways to study the biology of this obligate biotrophic pathogen. We started the analysis of the Bg tritici genome with the low-pass sequencing of its genome using the 454 technology and the construction of the first genomic bacterial artificial chromosome (BAC) library for this fungus. High-coverage contigs were assembled with the 454 reads. They allowed the characterization of 56 transposable elements and the establishment of the Blumeria repeat database. The BAC library contains 12,288 clones with an average insert size of 115 kb, which represents a maximum of 7.5-fold genome coverage. Sequencing of the BAC ends generated 12.6 Mb of random sequence representative of the genome. Analysis of BAC-end sequences revealed a massive invasion of transposable elements accounting for at least 85% of the genome.
Permanent Link: http://hdl.handle.net/11104/0203886